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At present we have four instruments in the Analytic and Preparative Cytometry Core:

Introducing the newest addition to our instrumentation:

BD LSRII Custom Designed Flow Cytometer

This instrument is equipped with Blue 488nm, Green 532nm, Red 633nm, and Violet 405nm lasers. The octagon and trigon optical arrays will enable detection of 2 colors off the blue, 5 colors off the green, 3 colors off the red, and 8 colors off the violet laser. Dichroic mirrors and bandpass filters are configured to detect the following dyes:

Blue laser: FITC, Alexa 488, PE

Green laser: PE CY7, PE CY5.5, PerCP CY5.5, PE Texas Red, PE

Red laser: APC, Alexa 700, APC CY7

Violet laser: Pacific Blue, AmCyan, Q dots

The acquisition/ analysis software is DiVa (PC based). Data can also be saved in FCS format and analysed using FlowJo software.

If a laboratory is interested in trying the instrument out, we suggest that one person be designated as the trainee. They will in turn train other members of the lab. We have identified some people who are familiar with the software. We will arrange training sessions as the need arises.
 

We also continue to maintain:

FACScan
This instrument is manufactured by Becton-Dickinson and contains a 488 nm argon laser.  It can detect up to three colors simultaneously such as FITC, PE, and PerCP.  It is connected to a MacIntosh computer  and utilizes "CellQuest" as the acquisition and analysis software.  This software is user-friendly and our  facility offers tutorials for those interested in learning to acquire/analyze their own samples.  Please call 934-1362 or email Enid.Keyser@ccc.uab.edu to schedule your tutorial.

FACSCalibur
This instrument is also manufactured by Becton-Dickinson and contains two lasers.  A 488 nm argon and a 633nm red diode laser enables this instrument to detect up to four colors simultaneously such as FITC,  PE, PerCP, and APC.  The FACSCalibur is also linked to a MacIntosh computer and uses "CellQuest" software.  If you would like more information on running four color experiments please call 934-1362 or  email Enid.Keyser@ccc.uab.edu.

FACSVantage SE with DiVa option
This instrument is also manufactured by Becton-Dickinson and has cell sorting capabilities.  It is equipped with two lasers, one coherent which emits at 488 nm and has a UV line which emits at 360 nm,  and one which is a HeNe emitting at 633 nm.  With the addition of the DiVa option we are capabale of running eight colors plus forward and side scatter parameters, as well as 2-way and 4-way sorting.  For more information on setting up a sorting experiment please call 934-1362 or email Enid.Keyser@ccc.uab.edu.